Leucine Dehydrogenase (LeuDH)
Nkọwa dị mkpirikpi:
Banyere Leucine dehydrogenase
Enwere ụdị ngwaahịa enzyme LeuDH 1 (Ọnụ ọgụgụ dị ka ES-LeuDH-101) nke SyncoZymes mepụtara.LeuDH nwere ike kpalite ammoniation reductive nke 4-methylbutanoic acid (nnu) na L-leucine ma ọ bụ oxidation nke leucine na 4-methylbutanoic acid.ES-LeuDH nwekwara ike kpalite mbelata amination nke α-keto acid nwere usoro yiri nke ahụ iji kwado usoro amino acid kwekọrọ.Na usoro nke catalysis, NADH dị mkpa dị ka hydrogen transporter.
Ụdị mmeghachi omume nke Leucine dehydrogenasecatalytic
Mobile/Wechat/WhatsApp: +86-13681683526
E-mail:lchen@syncozymes.com
| Enzymes | Koodu ngwaahịa | Nkọwapụta |
| Enzyme ntụ ntụ | ES-LeuDH-101 | Otu 1 Monoamine Oxidase, 50 mg nke ọ bụla 1 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ. |
Enwere ike iji usoro mmeghachi omume ndị a maka ntụnye aka:
1-10 mg / ml: mkpụrụ
10 mg / ml: enzymes
10% (v/v): Organic cosolvent (1)
100 mm: ihe nchekwa phosphate (pH7.0)
0.2 mg / ml: NAD +
100 mm: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Tụgharịa mmeghachi omume ahụ n'ọgba ọkụ mgbe niile (dịka ọmụmaatụ, 30oC, 150rpm) n'ime awa 24 ~ 48.Centrifuge ngwakọta ọ bụla iji kpalite protein, ha nwere ike iji ya mee ihe maka nyocha TLC, HPLC ma ọ bụ GC.
Rịba ama (1): Ihe mgbaze organic (dị ka DMSO, methanol, acetonitrile, IPA, wdg) bụ nhọrọ, ejiri mee ihe na-adịghị edozi.
