Leucine Dehydrogenase (LeuDH)
Nkọwa Dị Mkpirikpi:
Banyere Leucine dehydrogenase
E nwere otu ụdị ngwaahịa enzyme LeuDH (Nọmba dị ka ES-LeuDH-101) nke SyncoZymes mepụtara. LeuDH nwere ike ime ka ammonia nke 4-methylbutanoic acid (nnu) dị na L-leucine ma ọ bụ oxidation nke leucine gaa na 4-methylbutanoic acid. ES-LeuDH nwekwara ike ime ka ammonia nke α-keto acid dị na nhazi yiri ya dị na nkwadebe ihe ndị sitere na amino acid kwekọrọ. N'usoro catalysis, NADH dị mkpa dị ka ihe na-ebuga hydrogen.
Ụdị mmeghachi omume Leucine dehydrogenasecatalytic
Mkpanaka/Wechat/WhatsApp: +86-13681683526
Ozi-e:lchen@syncozymes.com
| Enzymes | Koodu Ngwaahịa | Nkọwapụta |
| Ntụ ntụ Enzyme | ES-LeuDH-101 | otu 1 Monoamine Oxidase, 50 mg nke ọ bụla 1 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ |
Enwere ike iji usoro mmeghachi omume a mee ihe maka ntụaka:
1-10 mg/ml: Ihe mejupụtara
10 mg/ml: Enzyme
10% (v/v): Ihe na-agbaze ihe dị ndụ (1)
100 mM: Ihe nchekwa phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ọdịdị Ammonium
10 mg/ml: Formate dehydrogenase (FDH)
Tinye mmeghachi omume ndị ahụ na igwe na-eme ka okpomọkụ dị jụụ (dịka ọmụmaatụ, 30oC, 150rpm) ruo awa 24-48. Tinye centrifuge ngwakọta ọ bụla iji mee ka protein dị ọhụrụ, enwere ike iji ihe na-eme ka ọ dị elu mee nyocha TLC, HPLC ma ọ bụ GC.
Rịba ama (1): Ihe mgbaze organic (dịka DMSO, methanol, acetonitrile, IPA, wdg) bụ nhọrọ, a na-eji ya maka ihe ndị na-anaghị agbaze agbaze.
