CALB anaghị arụ ọrụ
CALB anaghị emegharị ahụ site na mgbasa ozi anụ ahụ na resin hydrophobic nke ukwuu bụ macroporous, styrene/methacrylate polymer.CALB na-adịghị ahụkebe dabara adaba maka ngwa n'ime ihe mgbaze organic na sistemụ enweghị ihe mgbaze, enwere ike ịmegharị ya ma jiri ya mee ihe ọtụtụ oge n'ọnọdụ dabara adaba.
Koodu ngwaahịa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★ Higher ọrụ, elu chiral selectivity na elu kwụsie ike.
★ Mma arụmọrụ na-abụghị aqueous n'ụzọ.
★ mfe wepụ si mmeghachi omume usoro, ngwa ngwa kwụsị mmeghachi omume, ma zere protein fọdụrụnụ na ngwaahịa.
★ Enwere ike megharịa ya ma jiri ya belata ọnụ ahịa ya.
| Ihe omume | ≥10000PLU/g |
| pH nso maka mmeghachi omume | 5-9 |
| Oke okpomọkụ maka mmeghachi omume | 10-60 ℃ |
| Ọdịdị | CALB-IMMO100-A: Ìhè edo edo ruo aja aja siri ike CALB-IMMO100-B: Ọcha na-acha aja aja siri ike |
| Nha urughuru | 300-500μm |
| Ọnwụ na ihicha na 105 ℃ | 0.5% -3.0% |
| Resin maka immobilization | Macroporous, styrene/methacrylate polymer |
| Ihe mgbaze mmeghachi omume | Mmiri, ihe mgbaze organic, wdg, ma ọ bụ na-enweghị ihe mgbaze.Maka mmeghachi omume na ụfọdụ ihe mgbaze organic, 3% mmiri nwere ike ịgbakwunye iji melite mmetụta mmeghachi omume |
| Nha urughuru | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Nkọwa nkeji: 1 nkeji kwekọrọ na njikọ nke 1μmol kwa nkeji propyl laurate sitere na lauric acid na 1-propanol na 60 ℃.CALB-IMMP100-A na CALB-IMMO100-B nke dị n'elu kwekọrọ na ndị na-ebu ụgbọ ala nwere oke urughuru dị iche iche.
1. Reactor ụdị
Enzyme na-adịghị ahụkebe na-adabara ma kettle batch reactor na ihe na-eme ihe na-aga n'ihu na-aga n'ihu.Ekwesiri iburu n'uche ka o zere ikpochapu n'ihi ike nke ozo n'oge nri ma obu njuputa.
2. mmeghachi omume pH, okpomọkụ na ihe mgbaze
Ekwesịrị ịgbakwunye enzyme ahụ na-adịghị ahụkebe nke ikpeazụ, mgbe agbakwunyere ihe ndị ọzọ na agbaze, ma gbanwee pH.
Ọ bụrụ na oriri nke mkpụrụ ma ọ bụ imepụta ngwaahịa ga-eduga na mgbanwe pH n'oge mmeghachi omume, a ga-agbakwunye ihe nchekwa zuru ezu na usoro mmeghachi omume, ma ọ bụ ekwesịrị nyochaa ma mezie pH n'oge mmeghachi omume.
N'ime oke nnabata okpomọkụ nke CALB (n'okpuru 60 ℃), ọnụego mgbanwe mụbara na mmụba nke okpomọkụ.Na ojiji bara uru, a ga-ahọrọ okpomọkụ mmeghachi omume dịka nkwụsi ike nke mkpụrụ ma ọ bụ ngwaahịa.
N'ozuzu, mmeghachi omume ester hydrolysis dabara na sistemụ mmiri mmiri, ebe mmeghachi omume ester dabara adaba na sistemu akụkụ nke organic.Ihe mgbaze organic nwere ike ịbụ ethanol, tetrahydrofuran, n-hexane, n-heptane na toluene, ma ọ bụ ihe mgbaze agwakọta kwesịrị ekwesị.Maka mmeghachi omume na ụfọdụ ihe mgbaze organic, 3% mmiri nwere ike ịgbakwunye iji melite mmetụta mmeghachi omume.
3. Ejigharị na ndụ ọrụ nke CALB
N'okpuru ọnọdụ mmeghachi omume kwesịrị ekwesị, CALB nwere ike nwetaghachi ma jiri ya mee ihe, na oge ngwa a kapịrị ọnụ dịgasị iche iche na ọrụ dị iche iche.
Ọ bụrụ na ejighachighị CALB ahụ eweghachitere ma ọ dị mkpa ka echekwa ya mgbe mgbake gasịrị, ọ dị mkpa ka a sachaa ya akpọnwụọ ma mechie ya na 2-8 ℃.
Mgbe ọtụtụ ugboro nke ijigharị, ọ bụrụ na mmeghachi omume na-ebelata ntakịrị, CALB nwere ike ịgbakwunye nke ọma ma nọgide na-eji ya.Ọ bụrụ na arụ ọrụ mmeghachi omume na-ebelata nke ukwuu, ọ dị mkpa ka edochie ya.
Ọmụmaatụ 1 (Aminolysis)(1):
Ọmụmaatụ 2 (Aminolysis)(2):
Ọmụmaatụ 3 (Mgbanaka oghere polyester synthesis)(3):
Ọmụmaatụ 4 (Transesterification, regioselective nke hydroxyl otu)(4):
Ọmụmaatụ 5 (Transesterification, Kinetic mkpebi nke agbụrụ alcohols)(5):
Ọmụmaatụ 6(Esterification, kinetic mkpebi nke carboxylic acid)(6):
Ọmụmaatụ 7 (Esterolysis, mkpebi kinetic)(7):
Ọmụmaatụ 8 (Hydrolysis of amides)(8):
Ọmụmaatụ 9 (Acylation of amines)(9):
Ọmụmaatụ 10(Mgbakwunye aza-Michael)(10):
1. Chen S, Liu F, Zhang K, na Tal.Tetrahedron Lett, 2016, 57: 5312-5314 .
2. Olah M, Boros Z, anszky GH, e tal.Tetrahedron, 2016, 72: 7249-7255 .
3. Nakaoki1 T, Mei Y, Miller LM, na Tal.Ind. Biotechnol, 2005, 1 (2): 126-134.
4. Pawar SV, Yadav G DJ Ind. Eng.Chem, 2015, 31: 335-342 .
5. Kamble MP, Shinde SD, Yadav G DJ Mol.Catal.B: Ozizi, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. Usoro Biochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, e tal.J. Mol.Catal.B: Ozizi, 2016, 130: 58-69.
8. Gavil′an AT, Castillo E, L′pez-Mungu'AJ Mol.Catal.B: Enzym, 2006, 41: 136-140.
9. Joubioux FL, Henda YB, Bridiau N, e tal.J. Mol.Catal.B: Enzym, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, e tal.Tetrahedron Lett, 2010, 51: 4455-4458.
