Amidase (AMD)
Enzymes:Bụ macromolecular biological catalysts, ọtụtụ enzymes bụ protein
Amụma:Catalyze hydrolysis nke dị iche iche endogenous na ala ọzọ aliphatic na aromatic amides site na-ebufe otu acyl na mmiri na mmepụta nke free acids na amonia.A na-eji hydroxamic acid na acid organic ndị ọzọ eme ihe dị ka ọgwụ n'ihi na ha bụ ihe mejupụtara ihe na-eto eto, ọgwụ nje na ihe mgbochi tumor.Enwere ike kewaa amidases n'ụdị R na ụdị S dị ka ihe na-eme ka stereoselectivity si dị.
Na mgbakwunye na catalyzing hydrolysis nke amides, amidase nwekwara ike kpalite mmeghachi omume mbufe acyl n'ihu ihe ndị na-emepụta ihe dị ka hydroxylamine.
Amidase nwere isi mmalite dị iche iche nwere nkọwapụta mkpụrụ dị iche iche, ụfọdụ n'ime ha nwere ike hydrolyze aromatic amides, ụfọdụ n'ime ha nwere ike hydrolyze aliphatic amides, na ụfọdụ hydrolyze α-ma ọ bụ ω-amino amides.Ọtụtụ n'ime amines nwere ezigbo ọrụ catalytic naanị maka acyclic ma ọ bụ aromatic aromatic dị mfe, mana maka aromatics siri ike, heterocyclic amides, ọkachasị amides nwere ortho substituents, na-adịkarị ala na ọrụ (naanị enzymes ole na ole na-egosipụta mmetụta catalytic ka mma).
Usoro catalytic:
| Enzymes | Koodu ngwaahịa | Koodu ngwaahịa |
| Enzyme ntụ ntụ | ES-AMD-101~ ES-AMD-119 | set nke 19 amidases, 50 mg nke ọ bụla 19 ihe * 50mg / ihe, ma ọ bụ ndị ọzọ nke ukwuu. |
| Ngwa nyocha (SynKit) | ES-AMD-1900 | set nke 19 amidases, 1 mg nke ọ bụla 19 ihe * 1mg / ihe |
★ High mkpụrụ Specificity.
★ Strong chiral selectivity.
★ High akakabarede arụmọrụ.
★ Obere site-ngwaahịa.
★ Ọnọdụ mmeghachi omume dị nro.
★ enyi na gburugburu ebe obibi.
➢ Ekwesịrị ịme nyocha nke enzyme maka mkpụrụ osisi akọwapụtara n'ihi nkọwapụta mkpụrụ, wee nweta enzyme nke na-eme ka mkpụrụ nke ebumnuche na-eji mmetụta catalytic kacha mma.
➢ Ya adịla mgbe ị na-akpakọrịta na oke ọnọdụ dị ka: elu okpomọkụ, elu / ala pH na organic mgbaze na elu itinye uche.
➢ Dị ka ọ na-adịkarị, usoro mmeghachi omume kwesịrị ịgụnye mkpụrụ, ngwọta nchekwa (PH kacha mma mmeghachi omume pH nke enzyme).Ngwakọta ihe dị ka hydroxylamine kwesịrị ịdị na sistemụ mmeghachi omume mbufe acyl.
➢ Ekwesịrị ịgbakwunye AMD ikpeazụ n'ime sistemu mmeghachi omume yana pH kacha mma na ọnọdụ okpomọkụ.
➢ AMDdị AMD niile nwere ọnọdụ mmeghachi omume kachasị mma, yabụ, a ga-enyocha nke ọ bụla n'ime ha n'otu n'otu.
Ọmụmaatụ 1(1):
Arụ ọrụ hydrolysis na ihe Amide dị iche iche
| Mkpụrụ | Ọrụ akọwapụtara μmols min-1mg-1 | Mkpụrụ | Ọrụ akọwapụtara μmols min-1mg-1 |
| Acetamide | 3.8 | ο- OH benzamide | 1.4 |
| Propionamide | 3.9 | p- OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο- Toluamide | 0.3 |
| Pentanamide | 22.0 | p- Toluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Emere mmeghachi omume ahụ na 50mM sodium phosphate buffer solution, pH 7.5, na 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Emere mmeghachi omume ahụ na 50mM sodium phosphate buffer solution, pH 7.5, na 70 ℃.
Mmetụta reagent metụtara: amides, 100 mM (benzamide, 10 mM);hydroxylamine na hydrazine, 400 mM;enzyme 0.9 μM.
Ọmụmaatụ 2(2):
Ọmụmaatụ 3(3):
1. D'Abusco AS, Ammendola S., et al.Extremophiles, 2001, 5:183-192 .
2. Guo FM, Wu JP, Yang LR, et al.Usoro Biochemistry, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.Bioorganic Chemistry, 2017, dị na ntanetị 7.
