Nitrile reductase (NRED)
Nkọwa dị mkpirikpi:
Banyere nitrile reductase
Enwere ụdị ngwaahịa enzyme NRED atọ (Ọnụ ọgụgụ dị ka ES-NRED101~ ES-NRED103) nke SyncoZymes mepụtara.NRED nwere ike ime ka mbelata nitrile otu amine.Na usoro nke catalysis, NADPH dị mkpa dị ka hydrogen transporter.
Nitrile reductasecatalytic mmeghachi omume ụdị
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E-mail:lchen@syncozymes.com
| Enzymes | Koodu ngwaahịa | Nkọwapụta |
| Nkọwapụta | ES- NRED -101~ ES- NRED -103 | Nhazi nke 3 nitrile Reductase, 50 mg nke ọ bụla 3 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ. |
Enwere ike iji usoro mmeghachi omume ndị a maka ntụnye aka:
1-10 mg / ml mkpụrụ
10 mg / ml nke enzymes
10% (v/v) ihe na-eku ume (1)
Ihe nchekwa 100mM Phosphate (pH7.0)
0.2 mg/ml NADP+
10 mg / ml nke glucose
2 mg/ml Glucose dehydrogenase (GDH)
Tụgharịa mmeghachi omume ahụ n'ọgba ọkụ mgbe niile (dịka ọmụmaatụ, 30oC, 150rpm) n'ime awa 24 ~ 48.Centrifuge ngwakọta nke ọ bụla iji kpalite protein, ha nwere ike iji ya mee ihe maka nyocha TLC, HPLC ma ọ bụ GC.
Rịba ama (1): Ihe mgbaze organic (dị ka DMSO, methanol, acetonitrile, IPA, wdg) bụ nhọrọ, ejiri mee ihe na-adịghị edozi.
