Esterase&Lipase (PLE&CALB)

Nkọwa dị mkpirikpi:

Banyere Esterase&Lipase

ES-PLEs: Otu klas nke hydrolases na-eme ka nhazi na nbiji nke ester bond.Ha nwere ike isonye na ọtụtụ ụdị mmeghachi omume biochemical, dị ka esterification, transesterification na hydrolysis.A na-eji ha eme ihe n'ọtụtụ ebe dị ka nhazi nri, ụlọ ọrụ kemịkalụ, biomedicine, ọgwụ chiral na ọgwụgwọ gburugburu ebe obibi.

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E-mail:lchen@syncozymes.com

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Banyere Esterase&Lipase:

Enwere ụdị ngwaahịa enzyme PLE 26 (Ọnụ ọgụgụ dị ka ES-PLE-101～ES-PLE-126) nke SyncoZymes mepụtara.Enwere ike iji ES-PLE mee ihe maka hydrolysis nke ogige aliphatic na ester, ma ọ bụ mkpebi regioselective na stereoselective iji synthesize acid chiral na usoro ha.

Ụdị mmeghachi omume catalytic:

Uru:

★ High mkpụrụ Specificity.
★ Strong chiral selectivity.
★ Ntughari dị elu.
★ Obere site-ngwaahịa.
★ Ọnọdụ mmeghachi omume dị nro.
★ enyi na gburugburu ebe obibi.

Ntuziaka maka ojiji:

➢ Dị ka ọ na-adịkarị, sistemụ mmeghachi omume kwesịrị ịgụnye mkpụrụ, ihe nchekwa nchekwa na ES-PLE.A na-eme esterification nke ụfọdụ ES-PLE n'ime akụkụ nke organic.
➢ Ụdị ES-PLE niile dabara na ọnọdụ mmeghachi omume dị iche iche kwesịrị ka a mụọ ya n'otu n'otu.

Ọmụmaatụ ngwa:

Ọmụmaatụ 1 (Biosynthesis nke Pregabalin Intermediate)⁽¹⁾:

Ọmụmaatụ 2⁽²⁾:

Ọmụmaatụ 3⁽³⁾:

Ọmụmaatụ 4⁽⁴⁾:

Nchekwa:

Debe afọ 2 n'okpuru -20 ℃.

Nlebara anya:

Ejila ọnọdụ dị oke egwu emekọrịta ihe dị ka: oke okpomọkụ, elu/obere pH.

Ntụaka:

1. Xu FX, Chen SY, Xu G, na Tal.Ngwa.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD na tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917

Nke gara aga: Epoxide hydrolase (EH)

Osote: Formate dehydrogenase (FDH)

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