Nucloside phosphoryalse (NP)

Nkọwa dị mkpirikpi:

Banyere nucleoside phosphoryalse

Enwere ụdị ngwaahịa enzyme NP atọ (Ọnụ ọgụgụ dị ka ES-NP-101~ ES-NP-103) nke SyncoZymes mepụtara.ES-NP-101 bụ purine nucleoside phosphorylase, ES-NP-102 na ES-NP-103 bụ pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase nwere ike imebi nucleosides n'ime ntọala na pentose phosphate.Nucleoside phosphorylase nwere ike kewaa n'ime purine nucleoside phosphorylase na pyrimidine nucleoside phosphorylase dị ka mmasị nke nucleoside bases.Purine nucleoside phosphorylase nwere ike metabolize adenosine ka adenine, inosine ka hypoxanthine, guanosine ka guanine, ma mepụta ribose phosphate n'otu oge.Pyrimidine nucleoside phosphorylase nwere ike metabolize uridine na uracil ma mepụta phosphate ribose.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụdị mmeghachi omume phosphoryalse nke Nucloside:

Ozi ngwaahịa:

Enzymes	Koodu ngwaahịa	Nkọwapụta
Enzyme ntụ ntụ	ES-NP-101~ ES-NP-103	Ntụpọ nke 3 Nuclosidephosporyalse, 50 mg nke ọ bụla 3 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ.

Usoro mmeghachi omume nke Nucloside phosphoryalse:

Enwere ike iji usoro mmeghachi omume ndị a maka ntụnye aka:
1-10 mg / ml mkpụrụ
10 mg / ml nke enzymes
Ihe nchekwa 100mM Phosphate (pH7.0)
Mee ka mmeghachi omume ahụ na-eme ka ọ dị ọkụ mgbe niile (dịka ọmụmaatụ, 30oC, 150rpm) n'ime awa 12 ~ 24.Centrifuge ngwakọta ọ bụla iji kpalite protein, ha nwere ike iji ya mee ihe maka nyocha TLC, HPLC ma ọ bụ GC.