Transaminase (ATA)
Enzymes: Bụ macromolecular biological catalysts, ọtụtụ enzymes bụ protein.
Transaminases: Otu klas nke enzymes na-eme ka amino na-ebufe n'etiti amino acid na keto acid.Transaminases bụ isi enzymes ndu na njikọ asymmetric na mkpebi agbụrụ nke chiral amines.
Enwere ike kewaa Aminotransferase na klaasị anọ dịka usoro na nhazi: Ⅰ, Ⅱ, Ⅲ na Ⅳ.ω-aminotransferases bụ nke klas II transaminases, nke a na-ejikarị na nkwadebe nke amines chiral na amino acid na-ekwekọghị n'okike, dị ka β-amino acid.
ω-aminotransferases: N'ọtụtụ ọnọdụ, ω-transaminase na-ezo aka na klas nke enzymes, nke catalytic amonia na-ebufe mmeghachi omume na-enweghị α-amino acid dị ka mkpụrụ ma ọ bụ ngwaahịa.
Usoro catalytic:
| Enzymes | Koodu ngwaahịa | Koodu ngwaahịa |
| Enzyme ntụ ntụ | ES-ATA-101~ ES-ATA-165 | set nke 65 ω-Transaminases, 50 mg nke ọ bụla 65 ihe * 50mg / ihe, ma ọ bụ ndị ọzọ nke ukwuu. |
| Ngwa nyocha (SynKit) | ES-ATA-6500 | set nke 65 ω-Transaminases, 1 mg nke ọ bụla 65 ihe * 1mg / ihe |
★ High mkpụrụ Specificity.
★ Strong chiral selectivity.
★ High akakabarede arụmọrụ.
★ Obere site-ngwaahịa.
★ Ọnọdụ mmeghachi omume dị nro.
★ enyi na gburugburu ebe obibi.
➢ Ekwesịrị ịme nyocha nke enzyme maka mkpụrụ osisi a kapịrị ọnụ n'ihi nkọwapụta mkpụrụ, wee nweta enzyme nke na-eme ka mkpụrụ nke ebumnuche na-eji mmetụta catalytic kacha mma.
➢ Ya adịla mgbe ị na-akpakọrịta na oke ọnọdụ dị ka: elu okpomọkụ, elu / ala pH na organic mgbaze na elu itinye uche.
Dị ka ọ na-adịkarị, usoro mmeghachi omume kwesịrị ịgụnye mkpụrụ, ngwọta nchekwa, amino onyinye (dị ka amino acid na 1-phenyl ethylamine) ma ọ bụ onye na-anabata ya (dị ka keto acids), coenzyme (PLP), cosolvent (dị ka DMSO).
➢ Ekwesịrị ịgbakwunye ATA ikpeazụ n'ime sistemụ mmeghachi omume, mgbe emezigharịrị pH na ọnọdụ okpomọkụ ka ọ bụrụ ọnọdụ mmeghachi omume.
➢ Ụdị ATA niile nwere ọnọdụ mmeghachi omume dị iche iche, yabụ, a ga-amụkwu nke ọ bụla n'ime ha n'otu n'otu.
Ọmụmaatụ 1 (njikọ nke Sitagliptin, njikọ asymmetric)(1):
Ọmụmaatụ 2 (Mexiletine, Nchikota nke mkpebi kinetic na njikọ asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Sayensị, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Akwụkwọ ozi Organic, 2009,11 (21): 4810-4812.
