Transaminase (ATA)
Enzymes: Ha bụ ihe na-akpali macromolecular biological catalyst, ọtụtụ enzymes bụ protein.
Transaminases: Otu ụdị enzymes nke na-eme ka amino na-ebufe n'etiti amino acid na keto acid. Transaminases bụ enzymes dị mkpa na mmepụta asymmetric na mkpebi agbụrụ nke chiral amines.
Enwere ike kewaa Aminotransferase n'ime klas anọ dịka usoro na nhazi si dị: Ⅰ, Ⅱ, Ⅲ na Ⅳ. ω-aminotransferases bụ nke klas nke abụọ transaminases, a na-ejikarị ha eme ihe n'ịkwadebe chiral amines na amino acid ndị na-adịghị ahụkebe, dị ka β-amino acids.
ω-aminotransferases: N'ọtụtụ oge, ω-transaminase na-ezo aka na klaasị nke enzymes, nke na-ebufe mmeghachi omume ammonia catalytic na-enweghị α-amino acid dị ka ihe ndabere ma ọ bụ ngwaahịa.
Usoro ihe mkpali:
| Enzymes | Koodu Ngwaahịa | Koodu Ngwaahịa |
| Ntụ ntụ Enzyme | ES-ATA-101~ ES-ATA-165 | otu 65 ω-Transaminases, 50 mg nke ọ bụla 65 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ |
| Ngwa Nlele (SynKit) | ES-ATA-6500 | otu 65 ω-Transaminases, 1 mg nke ọ bụla 65 ihe * 1mg / ihe |
★ Nkọwapụta ihe dị elu nke substrate.
★ Ịhọrọ chiral siri ike.
★ Ntugharị dị elu.
★ Ngwaahịa ndị na-adịghị mma.
★ Ọnọdụ mmeghachi omume dị nro.
★ Ọ dị mma maka gburugburu ebe obibi.
➢ A ga-enyocha enzyme maka ihe ndị dị na ya n'ihi na ha nwere nkọwapụta ihe dị na ya, wee nweta enzyme nke na-akwalite ihe ndị dị na ya nke nwere mmetụta kacha mma na-akpali akpali.
➢ Emetụla aka na ọnọdụ dị oke njọ dịka: oke okpomọkụ, oke pH dị elu/ala na ihe mgbaze organic nwere oke njupụta.
➢ Dịka ọ na-adịkarị, usoro mmeghachi omume kwesịrị ịgụnye ihe ndị dị n'ime ya, ihe ngwọta nchekwa, amino donor (dịka amino acid na 1-phenyl ethylamine) ma ọ bụ ihe nnata (dịka keto acid), coenzyme (PLP), cosolvent (dịka DMSO).
➢ A ga-etinye ATA ikpeazụ n'ime sistemụ mmeghachi omume, mgbe emechara pH na okpomọkụ ka ọ dabara na ọnọdụ mmeghachi omume ahụ.
➢ Ụdị ATA niile nwere ọnọdụ mmeghachi omume kachasị mma dị iche iche, yabụ a ga-amụkwu nke ọ bụla n'ime ha n'otu n'otu.
Ihe atụ nke 1(njikọta Sitagliptin, njikọ asymmetric)(1):
Ihe atụ nke abụọ (Mexiletine, Njikọta nke mkpebi kinetic na njikọta asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, na ndị ọzọ. Sayensị, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Akwụkwọ ozi Organic, 2009,11 (21): 4810-4812.
