Ene reductase (ERED)

Nkọwa Dị Mkpirikpi:

Banyere ene reductase

ES-EREDs: Ha so n'ezinụlọ enzymes odo ochie nke na-ebelata C=C na α, β-unsaturated compounds yana otu ndị na-amịkọrọ electron. A na-ejikarị ha eme ihe n'ọmụmụ ihe gbasara amino acid sitere na ya, ihe ndị na-arụ ọrụ n'ime ahụ, ihe ndị na-emepụta ọgwụ, ihe na-esi ísì ụtọ na ihe ndị na-eme ka ahụ dị nro.

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Ozi-e:lchen@syncozymes.com

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Banyere ene reductase:

ES-EREDs na-akwalite ụdị substrate dị iche iche n'ihi ọtụtụ ụdị substrate dị iche iche. N'ozuzu, C=C nke α, β-unsaturated compounds nwere otu ndị na-amịkọrọ electron (gụnyere ketone aldehyde, nitro groups, carboxylic acid, esters, anhydride, lactones, imines, wdg) na-ebelata ngwa ngwa site na ES-EREDs, mana njikọ abụọ na-anaghị arụ ọrụ anaghị arụ ọrụ.

E nwere ụdị ngwaahịa enzyme ERED iri anọ na isii (Nọmba dịka ES-ERED-101~ES-ERED-146) nke SyncoZymes mepụtara.

Usoro ihe mkpali:

Ozi Ngwaahịa:

Enzymes	Koodu Ngwaahịa	Nkọwapụta
Ntụ ntụ Enzyme	ES-ERED-101~ ES-ERED-146	otu 46 Ene Reductases, 50 mg nke ọ bụla 46 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ
Ngwa Nlele (SynKit)	ES-ERED-4600	otu 46 Ene Reductases, 50 mg nke ọ bụla 46 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ

Uru:

★ Nkọwapụta ihe dị elu nke substrate.
★ Ịhọrọ chiral siri ike.
★ Mgbanwe dị elu.
★ Ngwaahịa ndị na-adịghị mma.
★ Ọnọdụ mmeghachi omume dị nro.
★ Ọ dị mma maka gburugburu ebe obibi.
★ Nchekwa dị elu.

Ntuziaka maka ojiji:

➢ Dịka ọ na-adịkarị, usoro mmeghachi omume kwesịrị ịgụnye ihe ndị dị n'ime ya, ihe ngwọta nchekwa (pH mmeghachi omume kacha mma), coenzymes (NAD(H) ma ọ bụ NADP(H)), usoro mmeghari coenzyme (dịka glucose na glucose dehydrogenase) na ES-ERED.
➢ Enwere ike ịnwale ES-ERED niile n'otu n'otu na sistemụ mmeghachi omume dị n'elu ma ọ bụ site na iji Ngwa Nnyocha ERED (SynKit ERED).
➢ A ga-amụ ụdị ES-ERED niile dabara na ọnọdụ mmeghachi omume kachasị mma dị iche iche n'otu n'otu.
➢ Nnukwu ihe e ji etinye ihe n'ime ala ma ọ bụ ngwaahịa nwere ike igbochi ọrụ ES-ERED. Agbanyeghị, enwere ike ibelata mgbochi ahụ site na itinye ihe e ji etinye ihe n'ime ala n'otu oge.

Ihe Nlereanya Ngwa:

Ihe atụ 1(α,β-unsaturated aldehydes ma ọ bụ ketones)⁽¹⁾:

Ihe atụ nke 2(α,β-unsaturated carboxylic acid na ihe ndị sitere na ha)⁽²⁾:

Nchekwa:

Debe ya n'ime ebe dị jụụ ruo afọ 2 n'okpuru -20 ℃.

Nlebara anya:

Emetụla aka na ọnọdụ dị oke njọ dịka: oke okpomọkụ, oke pH dị elu/ala na oke ihe mgbaze organic.

Ntụaka:

1. Lucídio C, Fardelone J, Augusto R, na Tal. J.Mol.Catal.B:Ezim., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, na tal. .Org.Lett, 2007, 9(26): 5409-5411.

Nke gara aga: D-Amino acid dehydrogenase (D-AADH)

Osote: Epooxide hydrolase (EH)

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