CALB anaghị arụ ọrụ

Nkọwa Dị Mkpirikpi:

KALB

A na-emepụta Lipase B nke a na-akpọ Recombinant Lipase B nke sitere na Candida antarctica (CALB) site na ịgbaze mmiri n'ime mmiri na Pichia pastoris nke agbanwere mkpụrụ ndụ ihe nketa.

Enwere ike iji CALB mee ihe na mmiri ma ọ bụ organic phase catalytic esterification, esterolysis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis of amides, acylation of amines na mgbakwunye mmeghachi omume.

CALB nwere nnukwu nhọrọ chiral na nhọrọ ọnọdụ, yabụ enwere ike iji ya mee ihe n'ọtụtụ ebe na nhazi mmanụ, nri, ọgwụ, ịchọ mma na ụlọ ọrụ kemịkalụ ndị ọzọ.

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Ozi-e:lchen@syncozymes.com

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Nkọwa Ngwaahịa

Akara Ngwaahịa

CALB anaghị arụ ọrụ:

A na-eme ka CALB ghara ịrụ ọrụ site na mmịpụta anụ ahụ na resin hydrophobic nke ukwuu nke bụ polima styrene/methacrylate macroporous. CALB a na-anaghị arụ ọrụ nke ọma dabara adaba maka iji ya na ihe mgbaze organic na sistemụ na-enweghị ihe mgbaze, enwere ike imegharị ya ma jiri ya mee ihe ọzọ ọtụtụ oge n'ọnọdụ kwesịrị ekwesị.
Koodu ngwaahịa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Uru nke SZ-CALB- IMMO100:

★Mmega ahụ dị elu, nhọrọ chiral dị elu na nkwụsi ike dị elu.
★Arụmọrụ ka mma n'oge mmiri na-adịghị.
★Wepụ ya ngwa ngwa na sistemụ mmeghachi omume, kwụsị mmeghachi omume ngwa ngwa, ma zere ihe fọdụrụ na protein na ngwaahịa ahụ.
★Enwere ike imegharị ya ọzọ ma jiri ya mee ihe ọzọ iji belata ọnụ ahịa ya.

Usoro Ngwaahịa nke SZ-CALB- IMMO100:

Ọrụ	≥10000PLU/g
oke pH maka mmeghachi omume	5-9
Oke okpomọkụ maka mmeghachi omume	10-60℃
Ọdịdị	CALB-IMMO100-A: Ọcha dị mfe site na odo ruo aja aja siri ike CALB-IMMO100-B: Ọcha ruo aja aja dị mfe
Nha nke ihe ndị dị ndụ	300-500μm
Ọnwụ mgbe akọrọ na 105℃	0.5%-3.0%
Resin maka immobilization	Polomita dị oke ọnụ, styrene/methacrylate
Ihe mgbaze mmeghachi omume	Mmiri, ihe mgbaze organic, wdg., ma ọ bụ na-enweghị ihe mgbaze. Maka mmeghachi omume na ụfọdụ ihe mgbaze organic, enwere ike itinye mmiri 3% iji melite mmetụta mmeghachi omume.
Nha nke ihe ndị dị ndụ	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Nkọwa nkeji: 1 nkeji kwekọrọ na mmepụta nke propyl laurate 1μmol kwa nkeji site na lauric acid na 1-propanol na 60℃. CALB-IMMP100-A na CALB-IMMO100-B dị n'elu kwekọrọ na ndị na-ebu ihe na-adịghị arụ ọrụ nke nwere nha dị iche iche.

Ntuziaka na Ihe Mgbochi:

1. Ụdị ihe nrụpụta
Enzyme a na-anaghị arụ ọrụ nke ọma na-emetụta ma ihe na-eme kettle batch na ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe ghara ịdị mma. Ọ dị mkpa ka a ghara ịkpasu ya iwe n'ihi ike mpụga mgbe a na-enye ya nri ma ọ bụ na-ejupụta ya.
2. pH mmeghachi omume, okpomọkụ na ihe mgbaze
A ga-etinye enzyme ahụ a na-anaghị arụ ọrụ n'ime ya n'ikpeazụ, mgbe etinyere ma gbazee ihe ndị ọzọ, ma gbanwee pH.
Ọ bụrụ na oriri nke ihe e ji mee ihe ma ọ bụ nhazi nke ngwaahịa ga-ebute mgbanwe nke pH n'oge mmeghachi omume ahụ, a ga-agbakwunye nchekwa zuru oke na sistemụ mmeghachi omume ahụ, ma ọ bụghị ya, a ga-enyocha ma gbanwee pH ahụ n'oge mmeghachi omume ahụ.
N'ime oke nnabata okpomọkụ nke CALB (n'okpuru 60 ℃), ọnụego mgbanwe mụbara ka okpomọkụ na-abawanye. N'iji ya eme ihe, a ga-ahọrọ okpomọkụ mmeghachi omume dịka nkwụsi ike nke ihe ma ọ bụ ngwaahịa si dị.
N'ozuzu, mmeghachi omume hydrolysis nke ester dị mma na sistemụ mmiri mmiri, ebe mmeghachi omume mmepụta nke ester dị mma na sistemụ usoro organic. Ihe mgbaze organic nwere ike ịbụ ethanol, tetrahydrofuran, n-hexane, n-heptane na toluene, ma ọ bụ ihe mgbaze agwakọtara kwesịrị ekwesị. Maka mmeghachi omume na ụfọdụ ihe mgbaze organic, enwere ike itinye mmiri 3% iji melite mmetụta mmeghachi omume.
3. CALB ji ya eme ihe ọzọ na ndụ ọrụ ya
N'okpuru ọnọdụ mmeghachi omume kwesịrị ekwesị, enwere ike weghachite ma jiri CALB mee ihe ọzọ, oge ngwa akọwapụtara dịkwa iche iche na ọrụ dị iche iche.
Ọ bụrụ na a naghị eji CALB eme ihe mgbe niile ma ọ dị mkpa ka a chekwaa ya mgbe ọ gbakechara, ọ dị mkpa ka a saa ya ma kpọọ ya nkụ ma mechie ya na okpomọkụ 2-8 ℃.
Mgbe e jirichara ya ọtụtụ ugboro, ọ bụrụ na e belatara arụmọrụ mmeghachi omume ahụ ntakịrị, enwere ike itinye CALB nke ọma ma nọgide na-eji ya. Ọ bụrụ na arụmọrụ mmeghachi omume ahụ ebelata nke ukwuu, ọ dị mkpa ka a dochie ya.

Ihe Nlereanya Ngwa:

Ihe atụ nke 1 (Aminolysis)⁽¹⁾:

Ihe atụ nke abụọ (Aminolysis)⁽²⁾:

Ihe atụ nke atọ (Mmepụta polyester mepere mgbanaka)⁽³⁾:

Ihe atụ nke 4 (Transesterification, regioselective nke otu hydroxyl)⁽⁴⁾:

Ihe atụ nke 5 (Ngbanwe, mkpebi kinetic nke mmanya agbụrụ)⁽⁵⁾:

Ihe atụ nke 6(Ịkọwapụta, mkpebi kinetic nke carboxylic acid)⁽⁶⁾:

Ihe atụ nke 7 (Esterolysis, mkpebi kinetic)⁽⁷⁾:

Ihe atụ nke 8 (Hydrolysis nke amides)⁽⁸⁾:

Ihe atụ nke 9 (Ịgbanwe amine)⁽⁹⁾:

Ihe atụ nke 10 (mmeghachi omume mgbakwunye Aza-Michael)⁽¹⁰⁾:

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