Nitrile reductase (NRED)
Nkọwa Dị Mkpirikpi:
Banyere Nitrile Reductase
E nwere ụdị ngwaahịa enzyme NRED atọ (Nọmba dịka ES-NRED101~ ES-NRED103) nke SyncoZymes mepụtara. NRED nwere ike ime ka mbelata nke otu nitrile ka ọ bụrụ amine. N'ime usoro catalysis, a chọrọ NADPH dị ka ihe na-ebuga hydrogen.
Ụdị mmeghachi omume nitrile reductasecatalytic
Mkpanaka/Wechat/WhatsApp: +86-13681683526
Ozi-e:lchen@syncozymes.com
| Enzymes | Koodu Ngwaahịa | Nkọwapụta |
| Nkọwapụta | ES- NRED -101~ ES- NRED -103 | otu setịpụ nke Nitrile Reductase atọ, 50 mg nke ọ bụla 3 * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ |
Enwere ike iji usoro mmeghachi omume a mee ihe maka ntụaka:
1-10 mg/ml Ihe mejupụtara
Enzyme 10 mg/ml
10% (v/v) Ihe na-agwakọta ihe dị ndụ (1)
Ihe nchekwa phosphate 100 mM (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Tinye mmeghachi omume ndị ahụ na igwe na-eme ka okpomọkụ dị jụụ (dịka ọmụmaatụ, 30oC, 150rpm) ruo awa 24-48. Tinye centrifuge ngwakọta ọ bụla iji mee ka protein dị ọhụrụ, enwere ike iji ihe na-eme ka ọ dị elu mee nyocha TLC, HPLC ma ọ bụ GC.
Rịba ama (1): Ihe mgbaze organic (dịka DMSO, methanol, acetonitrile, IPA, wdg) bụ nhọrọ, a na-eji ya maka ihe ndị na-anaghị agbaze agbaze.
