Cytochrome P450 monooxygenase (CYP)

Nkọwa dị mkpirikpi:

Ihe gbasara CYP

ES-CYPs: Otu klas nke redox enzymes nwere mercaptan-heme dị ka ebe na-arụ ọrụ iji mee ka nnyefe oxygen na-eme.Ha bụ ikuku oxygenases ma chọọ heme iji mee ka oxygen rụọ ọrụ.

Cytochrome P450 monooxygenase catalyze oxidation, epioxidation na hydroxylation nke alkyl, hydroxylation na oxidation nke amonia, oxidation nke sọlfọ, dealkylation (oxygen, sọlfọ, amonia), oxidative dehydrogenation, deamination na dehalogenation.
Enwere ụdị 8 nke ngwaahịa cytochrome P450 monooxygenase (Ọnụ ọgụgụ dị ka ES-CYP-101～ES-CYP-108) nke SyncoZymes mepụtara.

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E-mail:lchen@syncozymes.com

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Banyere CYP:

Ụdị mmeghachi omume catalytic:

Ozi ngwaahịa:

Enzymes	Ngwa nyocha (SynKit)	Nkọwapụta
Enzyme ntụ ntụ	ES-CYP-101~ ES-CYP-108	set nke 8 Cytochrome P450 Monooxygenases, 50 mg nke ọ bụla 8 ihe * 50mg / ihe, ma ọ bụ ihe ndị ọzọ.
Ngwa nyocha (SynKit)	ES-CYP-800	set nke 8 Cytochrome P450 Monooxygenases, 1mg nke ọ bụla 8 ihe * 1mg / ihe.

Uru:

★ Broad substrate ụdịdị dị iche iche.
★ Ntughari dị elu.
★ Obere site-ngwaahịa.
★ Ọnọdụ mmeghachi omume dị nro.
★ enyi na gburugburu ebe obibi.

Ntuziaka maka ojiji:

Dị ka ọ na-adịkarị, sistemụ mmeghachi omume kwesịrị ịgụnye mkpụrụ, ihe nchekwa nchekwa (PH kacha mma mmeghachi omume pH nke enzyme), coenzyme (NAD (H) ma ọ bụ NADP (H)), sistemụ nrụpụta coenzyme (dịka glucose na glucose dehydrogenase) na ES-CYP.Enwere ike dochie usoro coenzyme na coenzyme site na hydrogen peroxide na sistemụ mmeghachi omume akụkụ.
➢ Ụdị ES-CYP niile dabara na ọnọdụ mmeghachi omume dị iche iche kwesịrị ka a mụọ ya n'otu n'otu.
➢ Ngwakọta itinye uche dị elu ma ọ bụ ngwaahịa nwere ike igbochi ọrụ ES-CYP.Agbanyeghị, enwere ike belata ihe mgbochi ahụ site na mgbakwunye nke mkpụrụ osisi.

Ọmụmaatụ ngwa:

Ọmụmaatụ 1⁽¹⁾:

Ọmụmaatụ 2⁽²⁾:

Ọmụmaatụ 3⁽³⁾:

Ọmụmaatụ 4⁽⁴⁾:

Nchekwa:

Debe afọ 2 n'okpuru -20 ℃.

Nlebara anya:

Ejila ọnọdụ dị oke egwu na-akpakọrịta dị ka: oke okpomọkụ, elu / ala pH na nnukwu ihe mgbaze organic.

Ntụaka:

1. Zaretzki J, Matlock M, na Swamidass S JJ Chem.Inf.Ụdị, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., na Tal.J. Am.Chem.Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., na De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK na Tal.Acta Crystallogr.2009, 65, 372.