Esterase na Lipase (PLE&CALB)
E nwere ụdị ngwaahịa enzyme PLE iri abụọ na isii (Nọmba dịka ES-PLE-101～ES-PLE-126) nke SyncoZymes mepụtara. Enwere ike iji ES-PLE mee hydrolysis nke aliphatic na ester compounds, ma ọ bụ mkpebi regioselective na stereoselective iji mepụta chiral acid na ihe ndị sitere na ha.
Ụdị mmeghachi omume catalytic:
★ Nkọwapụta ihe dị elu nke substrate.
★ Ịhọrọ chiral siri ike.
★ Mgbanwe dị elu.
★ Ngwaahịa ndị na-adịghị mma.
★ Ọnọdụ mmeghachi omume dị nro.
★ Ọ dị mma maka gburugburu ebe obibi.
➢ Dịka ọ na-adịkarị, usoro mmeghachi omume kwesịrị ịgụnye substrate, ihe ngwọta nchekwa na ES-PLE. A na-eme esterification nke ụfọdụ ES-PLEs n'oge organic.
➢ A ga-amụ ụdị ES-PLE niile dabara na ọnọdụ mmeghachi omume kachasị mma dị iche iche n'otu n'otu.
Ihe atụ nke 1 (Biosynthesis nke Pregabalin Intermediate)(1):
Ihe atụ nke abụọ(2):
Ihe atụ nke atọ(3):
Ihe atụ nke anọ(4):
Debe ya n'ime ebe dị jụụ ruo afọ 2 n'okpuru -20 ℃.
Emetụla aka na ọnọdụ dị oke njọ dịka: oke okpomọkụ, oke pH dị elu/ala.
1. Xu FX, Chen SY, Xu G, na ndị ọzọ. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD na tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
