Haịrọdaịna (HAM)
Nkọwa Dị Mkpirikpi:
Banyere Hydroaminase
E nwere ụdị ngwaahịa enzyme HAM abụọ (Nọmba dịka ES-HAM-101~ ES-HAM-102) nke SyncoZymes mepụtara. HAM nwere ike ime ka ammonia nke enoic acid ma ọ bụ ihe ndị sitere na ya pụta iji mepụta chiral amino acid. Enwere ike iji HAM mepụta chiral amino acid (ma ọ bụ chiral amines) site na enoic acid (ma ọ bụ alkenes). Onye na-enye Ammonia, dị ka mmiri ammonia ma ọ bụ nnu ammonium, dị mkpa.
Ụdị mmeghachi omume hydroaminase catalytic
Mkpanaka/Wechat/WhatsApp: +86-13681683526
Ozi-e:lchen@syncozymes.com
| Enzymes | Koodu Ngwaahịa | Nkọwapụta |
| Ntụ ntụ Enzyme | ES-HAM-101~ ES-HAM-102 | otu setịpụ nke Nuclosidephosphoryalse abụọ, 50 mg nke ọ bụla Ihe abụọ * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ |
Enwere ike iji usoro mmeghachi omume a mee ihe maka ntụaka:
1-10 mg/ml: Ihe mejupụtara
10 mg/ml: Enzyme
10% (v/v): Ihe na-agbaze ihe dị ndụ (1)
10% (v/v): Amonia
Gbanwee pH ka ọ bụrụ 9.0 tupu ị tinye enzyme, a ga-agbakwunye enzyme ahụ n'ikpeazụ. Tinye mmeghachi omume ahụ na igwe na-eme ka okpomọkụ dị jụụ (dịka ọmụmaatụ, 30oC, 150rpm) ruo awa 24-48. Tinye centrifuge ngwakọta ọ bụla iji mee ka protein dị n'ime ya, enwere ike iji ihe na-eme ka ọ dị n'elu mee ihe maka nyocha TLC, HPLC ma ọ bụ GC.
Rịba ama (1): Ihe mgbaze organic (dịka DMSO, methanol, acetonitrile, IPA, wdg) bụ nhọrọ, a na-eji ya maka ihe ndị na-anaghị agbaze agbaze.
