Nucloside phosphoryalse (NP)

Nkọwa Dị Mkpirikpi:

Banyere phosphoryalse nucleoside

E nwere ụdị ngwaahịa NP enzyme atọ (Nọmba dịka ES-NP-101~ ES-NP-103) nke SyncoZymes mepụtara. ES-NP-101 bụ purine nucleoside phosphorylase, ES-NP-102 na ES-NP-103 bụ pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase nwere ike ịgbari nucleosides ka ọ bụrụ ntọala na pentose phosphate. Enwere ike kewaa Nucleoside phosphorylase ka ọ bụrụ purine nucleoside phosphorylase na pyrimidine nucleoside phosphorylase dịka nhọrọ nke nucleoside bases si dị. Purine nucleoside phosphorylase nwere ike ịgbari adenosine ka ọ bụrụ adenine, inosine ka ọ bụrụ hypoxanthine, guanosine ka ọ bụrụ guanine, ma mepụta ribose phosphate n'otu oge. Pyrimidine nucleoside phosphorylase nwere ike ịgbari uridine ka ọ bụrụ uracil ma mepụta ribose phosphate.

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Ụdị mmeghachi omume Nucloside phosphoryalse:

Ozi Ngwaahịa:

Enzymes	Koodu Ngwaahịa	Nkọwapụta
Ntụ ntụ Enzyme	ES-NP-101~ ES-NP-103	otu setịpụ nke Nuclosidephosphoryalse atọ, 50 mg nke ọ bụla 3 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ

Usoro nyocha mmeghachi omume Nucloside phosphoryalse:

Enwere ike iji usoro mmeghachi omume a mee ihe maka ntụaka:
1-10 mg/ml Ihe mejupụtara
Enzyme 10 mg/ml
Ihe nchekwa phosphate 100 mM (pH7.0)
Tinye mmeghachi omume ndị ahụ na igwe na-eme ka okpomọkụ dị jụụ (dịka ọmụmaatụ, 30oC, 150rpm) ruo awa 12-24. Tinye centrifuge ngwakọta ọ bụla iji mee ka protein dị ọhụrụ, enwere ike iji ihe na-eme ka ọ dị elu mee nyocha TLC, HPLC ma ọ bụ GC.