Amidase (AMD)
Enzymes:Ha bụ macromolecular biological catalyst, ọtụtụ enzymes bụ protein.
Ihe dị n'etiti:Mee ka mmiri dị n'ime mmiri dị iche iche nke aliphatic na aromatic amides dị iche iche site n'ibufe otu acyl gaa na mmiri site na mmepụta nke free acids na ammonia. A na-eji hydroxamic acid na organic acid ndị ọzọ eme ihe dị ka ọgwụ n'ihi na ha bụ ihe mejupụtara ihe ndị na-eto eto, ọgwụ nje na ihe ndị na-egbochi etuto ahụ. Enwere ike kewaa amidases n'ime ụdị R na ụdị S dịka stereoselectivity catalyst si dị.
Na mgbakwunye na ịkwalite hydrolysis nke amides, amidase nwekwara ike ịkpalite mmeghachi omume mbufe acyl n'ihu ihe ndị dị n'ime ya dịka hydroxylamine.
Amidase nwere isi mmalite dị iche iche nwere ụdị dị iche iche nke substrate, ụfọdụ n'ime ha nwere ike hydrolyze aromatic amides naanị, ụfọdụ n'ime ha nwere ike hydrolyze aliphatic amides naanị, ụfọdụ hydrolyze α-ma ọ bụ ω-amino amides. Ọtụtụ n'ime amines nwere ezigbo ọrụ catalytic naanị maka acyclic ma ọ bụ aromatic amides dị mfe, mana maka aromatics dị mgbagwoju anya, heterocyclic amides, ọkachasị amides nwere ortho substituents, na-adịkarị obere ọrụ (naanị enzymes ole na ole na-egosipụta mmetụta catalytic ka mma).
Usoro ihe mkpali:
| Enzymes | Koodu Ngwaahịa | Koodu Ngwaahịa |
| Ntụ ntụ Enzyme | ES-AMD-101~ ES-AMD-119 | otu 19 amidases, 50 mg nke ọ bụla 19 ihe * 50mg / ihe, ma ọ bụ ọnụọgụ ndị ọzọ |
| Ngwa Nlele (SynKit) | ES-AMD-1900 | otu 19 amidases, 1 mg nke ọ bụla 19 ihe * 1mg / ihe |
★ Nkọwapụta ihe dị elu nke substrate.
★ Ịhọrọ chiral siri ike.
★ Ntugharị dị elu.
★ Ngwaahịa ndị na-adịghị mma.
★ Ọnọdụ mmeghachi omume dị nro.
★ Ọ dị mma maka gburugburu ebe obibi.
➢ A ga-enyocha enzyme maka ihe ndị dị na ya n'ihi na ha nwere nkọwapụta ihe dị na ya, wee nweta enzyme nke na-akwalite ihe ndị dị na ya nke nwere mmetụta kacha mma na-akpali akpali.
➢ Emetụla aka na ọnọdụ dị oke njọ dịka: oke okpomọkụ, oke pH dị elu/ala na ihe mgbaze organic nwere oke njupụta.
➢ Dịka ọ na-adịkarị, sistemụ mmeghachi omume kwesịrị ịgụnye substrate, ihe ngwọta nchekwa (PH mmeghachi omume kachasị mma nke enzyme). Ndị na-ejikọta ihe dị ka hydroxylamine kwesịrị ịnọ na sistemụ mmeghachi omume nnyefe acyl.
➢ A ga-etinye AMD ikpeazụ n'ime sistemụ mmeghachi omume yana pH na okpomọkụ mmeghachi omume kacha mma.
➢ Ụdị AMD niile nwere ọnọdụ mmeghachi omume kachasị mma dị iche iche, yabụ a ga-amụkwu nke ọ bụla n'ime ha n'otu n'otu.
Ihe atụ nke 1(1):
Ọrụ hydrolysis na amide substrates dị iche iche
| Ihe ndị dị n'okpuru ala | Ihe omume kpọmkwem μmols nkeji-1mg-1 | Ihe ndị dị n'okpuru ala | Ihe omume kpọmkwem μmols nkeji-1mg-1 |
| Acetamide | 3.8 | ο-OH-Benzamide | 1.4 |
| Propionamide | 3.9 | p-OH-Benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο-Tọluamide | 0.3 |
| Pentanamide | 22.0 | p-Tọluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Pikolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Emere mmeghachi omume ahụ na 50mM sodium phosphate buffer solution, pH 7.5, na 70 ℃.
| Amids | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Emere mmeghachi omume ahụ na 50mM sodium phosphate buffer solution, pH 7.5, na 70 ℃.
Ọnụọgụgụ ihe ndị metụtara ya: amides, 100 mM(benzamide, 10 mM); hydroxylamine na hydrazine, 400 mM; enzyme 0.9 μM.
Ihe atụ nke abụọ(2):
Ihe atụ nke atọ(3):
1. D'Abusco AS, Ammendola S., et al. Extremophiles, 2001, 5:183-192 .
2. Guo FM, Wu JP, Yang LR, na ndị ọzọ. Usoro Biochemistry, 2015, 50(8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, na ndị ọzọ. Bioorganic Chemistry, 2017, Dị n'ịntanetị 7.
